Use of bacterial strains antagonistic to Escherichia coli for biocontrol of spinach: A field trial

To counteract global food safety hazards related to raw consumption of ready-to-eat leafy vegetables, a method improve bacterial status using antagonistic bacteria was studied under field conditions. This is the first study identify potential Escherichia coli antagonists from native microbiota on green vegetables and evaluate their effect in an industrial production setting. Bacterial strains were isolated different types selected upon against E. vitro, out 295 tested strains, 37 showed effect. Four those coated separate treatments onto spinach seeds planted field. Both plants analyzed by Illumina MiSeq next generation sequencing (NGS), it seen that contained lower relative abundance plant human pathogenic genera. Higher β-diversity observed for samples treated with Bacillus coagulans LMG P-32205 B. P-32206 compared control, indicating have induced substantial changes leaves. A reduction Escherichia-Shigella two isolates (Pseudomonas cedrina P-32207 Pseudomonas punonenis P-32204) as developed into plants. Seeds inoculated (B. P-32206) had increased levels Lactobacillaceae, treatment resulted Pantoea (from 31.4 12.2%). These results encourage usage part solution reduce risk pathogens vegetables.